Journal: Journal of Animal Science and Biotechnology

Article Title: Establishment of cell lines with porcine spermatogonial stem cell properties

doi: 10.1186/s40104-020-00439-0

Figure Lengend Snippet: Primer sequences for the RT-PCR and q-PCR analyses

Article Snippet: The primary antibodies used were mouse anti-UCHL1 (1: 1,000; ab8189, Abcam), rabbit anti-PLZF (1: 1,000; ab104854, Abcam), mouse anti-THY1 (1: 1,000; ab205719, Abcam), rabbit anti-VASA (1: 1,000; ab13840, Abcam), rabbit anti-DAZL (1: 500; ab34139, Abcam), goat anti-CXCR4 (1: 500; ab1670, Abcam), rabbit anti-SV40 (1: 1,000; 15,729, Cell Signaling Technology), mouse anti-PCNA (1: 1,000; sc-56, Santa Cruz Biotechnology), mouse anti-β-actin (1: 3,000; CW0096, CWBIO), rabbit anti-KIT (1: 1,000; 3074, Cell Signaling Technology) and rabbit anti-STRA8 (1: 1,000; ab49602, Abcam).

Techniques:

Journal: Journal of Animal Science and Biotechnology

Article Title: Establishment of cell lines with porcine spermatogonial stem cell properties

doi: 10.1186/s40104-020-00439-0

Figure Lengend Snippet: Characterization of immortalized porcine SSCs. a RT-PCR assay showing the transcripts of UCHL1 , PLZF , THY1 , VASA , DAZL , CXCR4 , SV40 and PCNA in the immortalized SSCs from passage 4 (P4) to passage 12 (P12). The RNAs from 7-day-old porcine testes are used as controls, and GAPDH serves as a loading control. -RT: the negative control omitting reverse transcription but with PCR reaction. b Western blot assay showing the protein expression of UCHL1, THY1, SV40, PCNA, PLZF, VASA, DAZL and CXCR4 in the immortalized SSCs at different passages. The protein extracts from 7-day-old porcine testes are used as controls, and β-actin serves as a loading control. c Staining of Ttag-transduced SSCs (passage 20) on cytospin slides for UCHL1, PLZF, VASA, DAZL, CXCR4, SV40 and PCNA. NC: the negative control using the isotype IgG in place of the primary antibody. Bar = 50 μm

Article Snippet: The primary antibodies used were mouse anti-UCHL1 (1: 1,000; ab8189, Abcam), rabbit anti-PLZF (1: 1,000; ab104854, Abcam), mouse anti-THY1 (1: 1,000; ab205719, Abcam), rabbit anti-VASA (1: 1,000; ab13840, Abcam), rabbit anti-DAZL (1: 500; ab34139, Abcam), goat anti-CXCR4 (1: 500; ab1670, Abcam), rabbit anti-SV40 (1: 1,000; 15,729, Cell Signaling Technology), mouse anti-PCNA (1: 1,000; sc-56, Santa Cruz Biotechnology), mouse anti-β-actin (1: 3,000; CW0096, CWBIO), rabbit anti-KIT (1: 1,000; 3074, Cell Signaling Technology) and rabbit anti-STRA8 (1: 1,000; ab49602, Abcam).

Techniques: Reverse Transcription Polymerase Chain Reaction, Negative Control, Western Blot, Expressing, Staining

Journal: eLife

Article Title: Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids

doi: 10.7554/eLife.26575

Figure Lengend Snippet: Primary antibodies DOI: http://dx.doi.org/10.7554/eLife.26575.041

Article Snippet: CD90 , Novus Biologicals, UK , NBP2-37330 (clone 7E1B11) , Mouse , 1:200 , No , N/A , RRID: AB_2665376.

Techniques: Amplification, Transduction

Journal: Molecular Medicine Reports

Article Title: High hydrostatic pressure induces apoptosis of retinal ganglion cells via regulation of the NGF signalling pathway

doi: 10.3892/mmr.2019.10206

Figure Lengend Snippet: Identification and purity testing of retinal ganglion cells. Green is Thy1.1 immunofluorescence staining and blue is DAPI staining (magnification, ×100 and ×200).

Article Snippet: Cells separated from the petri dish wall were transferred to another petri dish coated with HRP-conjugated goat anti-rabbit IgM (1:300; cat no. ab97180; Abcam) and mouse anti-rat monoclonal antibody Thy1.1 (1:100; cat no. ab6721; Abcam) for 30 min at room temperature, following which the cells that separated from the petri dish wall were removed.

Techniques: Immunofluorescence, Staining

Journal: Organogenesis

Article Title: Potential of Autologous Progenitor Cells and Decellularized Porcine Artery Matrix in Construction of Tissue-engineered Vascular Grafts

doi: 10.1080/15476278.2021.1963603

Figure Lengend Snippet: Pericardial effusion-derived progenitor cells (PEPCs) expressed vascular differentiation surface markers at day-14 after implantation on a decellularized porcine pulmonary artery. First row showed positive control of CD105 (green) and CD90 (red) expressions on HUVECs after implanting the cells on the intimal surface at day-14. PEPCs grew and migrated better when implanted on the adventitia surface (second row) as compared with implantation on intimal (the 3rd row) and lateral surfaces (the 4th row). All PEPCs expressed CD105 and CD90 after implantation. arrows indicated cells merged with all markers. Bar = 50 µm.

Article Snippet: After washing with PBS, the tissue sections were incubated with fluorescent-conjugated secondary antibody (Alexa Fluor® 488 goat anti-rabbit IgG (H + L), ab150077 for CD90 while ab150113 goat anti-mouse IgG (H + L) was applied for CD105, Abcam, Cambridge, UK) for 1 h at room temperature.

Techniques: Derivative Assay, Positive Control

Journal: Cell Death Discovery

Article Title: Notch signaling in astrocytes mediates their morphological response to an inflammatory challenge

doi: 10.1038/s41420-019-0166-6

Figure Lengend Snippet: Antibodies used

Article Snippet: Thy-1 (against CD90) , Mouse , 1:500 , Bio-Rad (Formerly AbD Serotec).

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Footprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model

doi: 10.1186/s13287-025-04325-2

Figure Lengend Snippet: Primer sequences used in this study

Article Snippet: CD90 , primary , polyclonal , rabbit , human , Bioss [bs-0778R] , none , 1:200.

Techniques: Virus

Journal: Stem Cell Research & Therapy

Article Title: Footprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model

doi: 10.1186/s13287-025-04325-2

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: CD90 , primary , polyclonal , rabbit , human , Bioss [bs-0778R] , none , 1:200.

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Footprint-free induced pluripotent stem cells can be successfully differentiated into mesenchymal stromal cells in the feline model

doi: 10.1186/s13287-025-04325-2

Figure Lengend Snippet: Successful generation of feline iPSC-derived mesenchymal stromal cells (MSCs). ( A ). Morphology of iPSC-derived MSCs-1 and − 2, with evenly distributed plastic adherent cells. ( B ). Conventional RT-PCR analysis of feline MSC-specific surface markers CD29 , CD44 , CD73 , CD90 , and CD105 , the hematopoietic stem cell marker CD34 , and the loading control GAPDH , in feline iPSC-MSCs and iPSCs. Full-length gels are presented in Additional File 10: Fig. . ( C & D ). Immunofluorescence ( C ) and flow cytometric analysis ( D ) of MSC-specific feline surface proteins CD44, CD73, CD90, and CD105 (green). Nuclei were visualized by staining with DAPI (blue)

Article Snippet: CD90 , primary , polyclonal , rabbit , human , Bioss [bs-0778R] , none , 1:200.

Techniques: Derivative Assay, Reverse Transcription Polymerase Chain Reaction, Marker, Control, Immunofluorescence, Staining